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Proteomic Analysis of Hydrogen Photoproduction in Sulfur-Deprived Chlamydomonas Cells

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posted on 06.08.2010 by Mei Chen, Le Zhao, Yong-Le Sun, Su-Xia Cui, Li-Fang Zhang, Bin Yang, Jie Wang, Ting-Yun Kuang, Fang Huang
The green alga Chlamydomonas reinhardtii is a model organism to study H2 metabolism in photosynthetic eukaryotes. To understand the molecular mechanism of H2 metabolism, we used 2-DE coupled with MALDI-TOF and MALDI-TOF/TOF-MS to investigate proteomic changes of Chlamydomonas cells that undergo sulfur-depleted H2 photoproduction process. In this report, we obtained 2-D PAGE soluble protein profiles of Chlamydomonas at three time points representing different phases leading to H2 production. We found over 105 Coomassie-stained protein spots, corresponding to 82 unique gene products, changed in abundance throughout the process. Major changes included photosynthetic machinery, protein biosynthetic apparatus, molecular chaperones, and 20S proteasomal components. A number of proteins related to sulfate, nitrogen and acetate assimilation, and antioxidative reactions were also changed significantly. Other proteins showing alteration during the sulfur-depleted H2 photoproduction process were proteins involved in cell wall and flagella metabolisms. In addition, among these differentially expressed proteins, 11 were found to be predicted proteins without functional annotation in the Chlamydomonas genome database. The results of this proteomic analysis provide new insight into molecular basis of H2 photoproduction in Chlamydomonas under sulfur depletion.

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