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Microarray Based Screening of Peptide Nano Probes for HER2 Positive Tumor

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journal contribution
posted on 18.08.2015 by Zihua Wang, Weizhi Wang, Xiangli Bu, Zewen Wei, Lingling Geng, Yue Wu, Chengyan Dong, Liqiang Li, Di Zhang, Shu Yang, Fan Wang, Christopher Lausted, Leroy Hood, Zhiyuan Hu
Peptides are excellent biointerface molecules and diagnostic probes with many advantages such as good penetration, short turnover time, and low cost. We report here an efficient peptide screening strategy based on in situ single bead sequencing on a microarray. Two novel peptides YLFFVFER (H6) and KLRLEWNR (H10) specifically binding to the tumor biomarker human epidermal growth factor receptor 2 (HER2) with aKD of 10–8 M were obtained from a 105 library. Conjugated to nanoparticles, both the H6 and H10 probes showed specific accumulation in HER2-positive tumor tissues in xenografted mice by in vivo imaging.