Magnetic Susceptibility-Induced Alignment of Proteins in Reverse Micelles
journal contributionposted on 20.12.2006 by Kathleen G. Valentine, Maxim S. Pometun, Joseph M. Kielec, Robert E. Baigelman, Jayme K. Staub, Kristy L. Owens, A. Joshua Wand
Any type of content formally published in an academic journal, usually following a peer-review process.
Proteins encapsulated within the aqueous core of reverse micelles are found to partially align in a magnetic field. The degree of alignment is sufficient to result in sizable residual 15N−H dipolar couplings that can be easily measured. It is found that the magnetic susceptibility of the reverse micelle particle is not dominated by the encapsulated protein. The residual dipolar couplings are found to be structurally meaningful.