Intracellular Fluorescent Temperature Probe Based on Triarylboron Substituted Poly N‑Isopropylacrylamide and Energy Transfer
journal contributionposted on 07.04.2015 by Jun Liu, Xudong Guo, Rui Hu, Jian Xu, Shuangqing Wang, Shayu Li, Yi Li, Guoqiang Yang
Any type of content formally published in an academic journal, usually following a peer-review process.
A novel hydrophilic fluorescence temperature probe (PNDP) based on polarity-sensitive triarylboron compound (DPTB) and PNIPAM is designed and synthesized. In order to overcome the shortcomings of the single-intensity-based sensing mechanism and obtain more robust signals, ratiometric readout is achieved by designing an efficient FRET system (PNDP-NR) between DPTB and Nile Red (NR). PNDP-NR possesses some excellent features, including wide temperature range, good linear relationship, high temperature resolution, excellent reversibility, and stability. Within a sensing temperature range of 30–55 °C, the fluorescence color of PNDP-NR experiences significant change from red to green-blue. PNDP-NR is also introduced into NIH/3T3 cells to sense the temperature at the single-cell level. It gave excellent photostability and low cytotoxicity in vivo.