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Influence of the Allelic Variants Encoded at the Gli-B1 Locus, Responsible for a Major Allergen of Wheat, on IgE Reactivity for Patients Suffering from Food Allergy to Wheat

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posted on 07.02.2007 by Sandra Denery-Papini, Michel Lauriére, Gérard Branlard, Martine Morisset, Catherine Pecquet, Dominique Choudat, Marielle Merlino, Florence Pineau, Yves Popineau, Elodie Boulenc, Isabelle Bouchez-Mahiout, Marie Bodinier, Denise-Anne Moneret-Vautrin
Wheat presents an important genetic diversity that could be useful to look for cultivars with reduced allergencity. ω5-Gliadins have been described as major allergens for wheat allergic patients suffering from wheat-dependent exercise-induced anaphylaxis (WDEIA) and some cases of chronic urticaria (U). Our objective was to study the influence of genetic variability at the Gli-B1 locus encoding for ω5-gliadins on the reactivity of IgE antibodies from these patients. We selected cultivars expressing 13 alleles at Gli-B1 including a wheat/rye translocation and studied the reactivity to gliadins of a rabbit antiserum specific for ω5-gliadins and of IgE from 10 patients. The antiserum and IgE from nine patients with WDEIA and U strongly detected ω5-gliadins expressed by most of the Gli-B1 alleles but showed no or faint responses to the gliadins and secalins extracted from the translocated wheat. The selection of genotypes lacking the Gli-B1 locus may reduce wheat allergenicity. Keywords: Wheat; food allergy; exercise induced anaphylaxis; ω5-gliadins; allelic variants

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