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Identification of Novel and Cell Type Enriched Cofactors of the Transcription Activation Domain of RelA (p65 NF-κB)

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journal contribution
posted on 08.08.2005 by Heather R. Owen, Manfredo Quadroni, Willy Bienvenut, Christine Buerki, Michael O. Hottiger
RelA (NF-κB) is a transcription factor inducible by distinct stimuli in many different cell types. To find new cell type specific cofactors of NF-κB dependent transcription, we isolated RelA transcription activation domain binding proteins from the nuclear extracts of three different cell types. Analysis by electrophoresis and liquid chromatography tandem mass spectrometry identified several novel putative molecular partners. Some were strongly enriched in the complex formed from the nuclear extracts of specific cell types. Keywords: NF-κB • RelA • p65 • transcription • cofactors • mass spectrometry