Gram-Scale Synthesis and Biofunctionalization of Silica-Coated Silver Nanoparticles for Fast Colorimetric DNA Detection
journal contributionposted on 15.04.2005 by Shuhua Liu, Zhihua Zhang, Mingyong Han
Any type of content formally published in an academic journal, usually following a peer-review process.
A direct silica-coating method has been developed for the gram-scale synthesis of well-dispersed Ag@SiO2 nanoparticles. Subsequent surface functionalization via the well-established silica surface chemistry provided arching points for straightforward bioconjugation with amino-terminated oligonucleotides. Fast hybridization kinetics of the resulting robust oligo-modified Ag@SiO2 nanoprobes with complementary target oligonucleotides render themselves very useful for the fast colorimetric DNA detection based on the sequence-specific hybridization properties of DNA. Additionally, the reliable protocols developed in this study for preparing and functionalizing Ag@SiO2 nanoparticles can be readily extended to other silica-coated nanoparticles, which can also provide a specific platform for the covalent attachment of biomolecules such as amino-rich proteins, enzymes, or amino-terminated oligonucleotides for diverse bioapplications.