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Glyconanoparticle Aided Detection of β‑Amyloid by Magnetic Resonance Imaging and Attenuation of β‑Amyloid Induced Cytotoxicity

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posted on 17.04.2013 by Hovig Kouyoumdjian, David C. Zhu, Mohammad H. El-Dakdouki, Kelly Lorenz, Jianjun Chen, Wei Li, Xuefei Huang
The development of a noninvasive method for the detection of Alzheimer’s disease is of high current interest, which can be critical in early diagnosis and in guiding treatment of the disease. The aggregates of β-amyloid are a pathological hallmark of Alzheimer’s disease. Carbohydrates such as gangliosides have been shown to play significant roles in initiation of amyloid aggregation. Herein, we report a biomimetic approach using superparamagnetic iron oxide glyconanoparticles to detect β-amyloid. The bindings of β-amyloid by the glyconanoparticles were demonstrated through several techniques including enzyme linked immunosorbent assay, gel electrophoresis, tyrosine fluorescence assay, and transmission electron microscopy. The superparamagnetic nature of the nanoparticles allowed easy detection of β-amyloid both in vitro and ex vivo by magnetic resonance imaging. Furthermore, the glyconanoparticles not only were nontoxic to SH-SY5Y neuroblastoma cells but also greatly reduced β-amyloid induced cytotoxicity to cells, highlighting the potential of these nanoparticles for detection and imaging of β-amyloid.

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