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Dichlororesorufin-Based Colorimetric and Fluorescent Probe for Ultrasensitive Detection of Mercury Ions in Living Cells and Zebrafish

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journal contribution
posted on 10.12.2018 by Qingxia Duan, Xiaoyu Lv, Caiyun Liu, Zhuofan Geng, Fenfen Zhang, Wenlong Sheng, Zuokai Wang, Pan Jia, Zilu Li, Hanchuang Zhu, Baocun Zhu
In this work, a simple dichlororesorufin-based colorimetric and fluorescent probe R-Hg with the recognition receptor of carbonothioate was designed, synthesized, and exploited to determine mercury ions in aqueous solution and living cells as well as in zebrafish. After a series of analytical tests, probe R-Hg showed some excellent characteristics, such as ultrasensitivity, high selectivity, and good water solubility. The probe solution exhibited a very large fluorescence enhancement (about 150-fold) upon addition of Hg2+. Additionally, probe R-Hg exhibited excellent sensitivity, which could quantificationally detect Hg2+ at the nanomolar level, and the detection limit was 0.49 nM. Moreover, probe R-Hg could be used as a “naked-eye” probe for monitoring Hg2+, because the color of its solution transformed remarkably from yellow into pink with the participation of Hg2+. Importantly, probe R-Hg has potential application value and has been successfully applied to the determination of Hg2+ in practical water samples and in living RAW 264.7 cells as well as in zebrafish as a bioimaging reagent.