Breaking the Degeneracy of the Genetic Code
journal contributionposted on 29.05.2003 by Inchan Kwon, Kent Kirshenbaum, David A. Tirrell
Any type of content formally published in an academic journal, usually following a peer-review process.
A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.