ja0350076_si_001.pdf (697.33 kB)

Breaking the Degeneracy of the Genetic Code

Download (697.33 kB)
journal contribution
posted on 29.05.2003 by Inchan Kwon, Kent Kirshenbaum, David A. Tirrell
A mutant yeast phenylalanine transfer RNA (ytRNAPheAAA) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNAPheAAA and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.