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Boron Binding with the Quorum Sensing Signal AI-2 and Analogues

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journal contribution
posted on 22.07.2004 by Martin F. Semmelhack, Shawn R. Campagna, Charlotte Hwa, Michael J. Federle, Bonnie L. Bassler
The unstable bacterial metabolic product, DPD, and the related natural product, laurencione, are shown to have a high affinity for borate complexation, through the hydrated analogue. The boron complex of DPD is Vibrio harveyi AI-2, an interspecies quorum sensing signal in bacteria, and an affinity column with a borate resin is effective in providing the first method for concentrating and purifying V. harveyi AI-2 from the biosynthetic product.