Assessing the Utility of Infrared Spectroscopy as a Structural Diagnostic Tool for β‑Sheets in Self-Assembling Aromatic Peptide Amphiphiles
journal contributionposted on 30.07.2013 by Scott Fleming, Pim W. J. M. Frederix, Iván Ramos Sasselli, Neil T. Hunt, Rein V. Ulijn, Tell Tuttle
Any type of content formally published in an academic journal, usually following a peer-review process.
β-Sheets are a commonly found structural motif in self-assembling aromatic peptide amphiphiles, and their characteristic “amide I” infrared (IR) absorption bands are routinely used to support the formation of supramolecular structure. In this paper, we assess the utility of IR spectroscopy as a structural diagnostic tool for this class of self-assembling systems. Using 9-fluorene-methyloxycarbonyl dialanine (Fmoc-AA) and the analogous 9-fluorene-methylcarbonyl dialanine (Fmc-AA) as examples, we show that the origin of the band around 1680–1695 cm–1 in Fourier transform infrared (FTIR) spectra, which was previously assigned to an antiparallel β-sheet conformation, is in fact absorption of the stacked carbamate group in Fmoc-peptides. IR spectra from 13C-labeled samples support our conclusions. In addition, DFT frequency calculations on small stacks of aromatic peptides help to rationalize these results in terms of the individual vibrational modes.