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Application of a High Throughput Bioluminescence-Based Method and Mathematical Model for the Quantitative Comparison of Polymer Microbicide Efficiency

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journal contribution
posted on 11.05.2009 by Thomas R. Stratton, R. Edwin García, Bruce M. Applegate, Jeffrey P. Youngblood
Quaternized poly(4-vinyl pyridine)-based copolymers are known to be effective against a wide range of bacteria and possess biocompatible properties. Extensive testing of a wide range of copolymers is necessary to further explore and enhance the biocidal properties. However, testing is hampered by labor-intensive bacteria testing techniques. The present paper presents a new testing method, based on bioluminescent reporter strains to enable fast evaluation of bactericidal properties. The reported method enables us to create real-time characterization of bacteria behavior with far less labor than required through traditional testing methods. A mathematical model was also developed to characterize the change in bacteria populations exposed to biocides and to enable the quantitative comparison of minimum bactericidal concentrations.