Application of a High Throughput Bioluminescence-Based Method and Mathematical Model for the Quantitative Comparison of Polymer Microbicide Efficiency
journal contributionposted on 11.05.2009 by Thomas R. Stratton, R. Edwin García, Bruce M. Applegate, Jeffrey P. Youngblood
Any type of content formally published in an academic journal, usually following a peer-review process.
Quaternized poly(4-vinyl pyridine)-based copolymers are known to be effective against a wide range of bacteria and possess biocompatible properties. Extensive testing of a wide range of copolymers is necessary to further explore and enhance the biocidal properties. However, testing is hampered by labor-intensive bacteria testing techniques. The present paper presents a new testing method, based on bioluminescent reporter strains to enable fast evaluation of bactericidal properties. The reported method enables us to create real-time characterization of bacteria behavior with far less labor than required through traditional testing methods. A mathematical model was also developed to characterize the change in bacteria populations exposed to biocides and to enable the quantitative comparison of minimum bactericidal concentrations.