pr7b00450_si_002.xlsx (456.63 kB)
iTRAQ-Based Proteomic Analysis Reveals Recovery of Impaired Mitochondrial Function in Ischemic Myocardium by Shenmai Formula
dataset
posted on 2018-01-04, 00:00 authored by Yi Wang, Yu Zhao, Wei Jiang, Xiaoping Zhao, Guanwei Fan, Han Zhang, Peiqiang Shen, Jiangmin He, Xiaohui FanShenmai formula (SM)
has been a traditional medicinal remedy for
treating cardiovascular diseases in China for 800 years; however,
its mechanism of action remains unclear. To explore the mechanism
underlying cardioprotective effects of SM, iTRAQ-based proteomic approach
was applied to analyze protein of myocardium in rats with myocardial
ischemic injury. Upon treatment with SM and its two major components
Red ginseng (RG) and Radix Ophiopogonis (OP), 101 differentially expressed
proteins were filtered from a total of 712 detected and annotated
proteins. They can be classified according to their locations and
functions, while most of them are located in intracellular organelle,
participating in cellular metabolic process. The functions of them
are mostly associated with mitochondrial oxidative phosphorylation/respiration.
The differentially expressed proteins were validated by liquid chromatography–tandem
mass spectrometry and Western blotting (ATP5D, NDUFB10, TNNC1). Further in vitro experiments found that SM could attenuate hypoxia
induced impairment of mitochondrial membrane potential and cellular
ATP concentration in neonatal rat ventricular myocytes. Interestingly,
the result of quantitative mitochondrial biogenesis assays revealed
that SM had dominant positive effects on the maximum respiration,
ATP-coupled respiration, and spare capacity of mitochondria in response
to hypoxia. Hence, our findings suggest that SM promotes mitochondrial
function to protect cardiomyocytes against hypoxia, which provides
a possible illustration for conventional botanical therapy on a molecular
level.
History
Usage metrics
Keywords
5DRGIschemic MyocardiumShenmai Formula Shenmai formulaATP concentration800 yearsRadix Ophiopogoniscomponents Red ginsengiTRAQ-Based Proteomic AnalysisImpaired Mitochondrial Functionattenuate hypoxiamitochondrial membraneischemic injuryOPmitochondrial biogenesis assaysmitochondrial functioncardioprotective effectsintracellular organelleSMiTRAQ-based proteomic approachproteinTNNCNDUFBATP-coupled respiration
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC