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Toward Hypoxia-Selective Rhenium and Technetium Tricarbonyl Complexes

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posted on 17.12.2015, 09:58 by Andrea J. North, David J. Hayne, Christine Schieber, Katherine Price, Anthony R. White, Peter J. Crouch, Angela Rigopoulos, Graeme J. O’Keefe, Henri Tochon-Danguy, Andrew M. Scott, Jonathan M. White, Uwe Ackermann, Paul S. Donnelly
With the aim of preparing hypoxia-selective imaging and therapeutic agents, technetium­(I) and rhenium­(I) tricarbonyl complexes with pyridylhydrazone, dipyridylamine, and pyridylaminocarboxylate ligands containing nitrobenzyl or nitroimidazole functional groups have been prepared. The rhenium tricarbonyl complexes were synthesized with short reaction times using microwave irradiation. Rhenium tricarbonyl complexes with deprotonated p-nitrophenyl pyridylhydrazone ligands are luminescent, and this has been used to track their uptake in HeLa cells using confocal fluorescent microscopy. Selected rhenium tricarbonyl complexes displayed higher uptake in hypoxic cells when compared to normoxic cells. A 99mTc tricarbonyl complex with a dipyridylamine ligand bearing a nitroimidazole functional group is stable in human serum and was shown to localize in a human renal cell carcinoma (RCC; SK-RC-52) tumor in a mouse.