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The PI(3,5)P2 and PI(4,5)P2 Interactomes

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posted on 2008-12-05, 00:00 authored by Bruno Catimel, Christine Schieber, Melanie Condron, Heather Patsiouras, Lisa Connolly, Jenny Catimel, Edouard C. Nice, Antony W. Burgess, Andrew B. Holmes
A comprehensive analysis of the phosphoinositide interactome has been performed using analogues of PI(3,5)P2 and PI(4,5)P2 phosphatidyl phospholipids which were immobilized onto Affi-10 beads or incorporated into liposomes for use as affinity absorbents with cytosolic extracts from colonic carcinoma cell lines. Affinity/LC/MS/MS experiments allowed identification of 388 proteins/protein complexes that appeared to interact specifically with the phosphoinositide targets: a number of novel potential phosphoinositide interacting proteins have been identified.

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