jf8b02708_si_002.zip (4.62 MB)
Download file

Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata

Download (4.62 MB)
posted on 27.08.2018, 00:00 by Xianping Fang, Ya Xin, Zheliang Sheng, Hui Liu, Aili Jiang, Fang Wang, Jian Yang, Xiaojun Xi, Qian Zha, Liqing Zhang, Liangying Dai, Chengqi Yan, Jianping Chen
The various post-translational modifications (PTMs) of plant proteins have important regulatory roles in development. We therefore examined various modified proteins from strawberry stigmata and found that succinylation of lysine residues was the most abundant type of modification. We then subjected proteins from strawberry stigmata to an efficient enrichment method for succinylated peptides and identified 200 uniquely succinylated lysines (Suks) in 116 proteins. A bioinformatics analysis revealed that these proteins are involved in important biological processes, including stress responses, vesicular transport, and energy metabolism. Proteomics, combined with immunoprecipitation and immunoblotting, revealed an obvious increase in succinylation of the assembly polypeptide 2 (AP2) and clathrin from 0.5 to 2 h after pollination, suggesting that succinylation is involved in the recognition of pollen–stigma signaling substances and vesicular transport. These results suggest that AP2/clathrin-mediated vesicular transport processes are regulated by lysine succinylation during pollen recognition.