posted on 2018-07-02, 00:00authored byGuangle Niu, Ruoyao Zhang, John P. C. Kwong, Jacky W. Y. Lam, Congping Chen, Jianguo Wang, Yuncong Chen, Xing Feng, Ryan T. K. Kwok, Herman H.-Y. Sung, Ian D. Williams, Mark R. J. Elsegood, Jianan Qu, Chao Ma, Kam Sing Wong, Xiaoqiang Yu, Ben Zhong Tang
Lipid
droplets are highly associated with obesity, diabetes, inflammatory
disorders, and cancer. A reliable two-photon dye for specific lipid
droplets imaging in live cells and live tissues at ultralow concentration
has rarely been reported. In this work, four new aggregation-induced
emission luminogens (AIEgens) based on the naphthalene core were designed
and synthesized for specific two-photon lipid droplet staining. The
new molecules, namely, NAP AIEgens, exhibit large Stokes shift (>110
nm), high solid-state fluorescence quantum yield (up to 30%), good
two-photon absorption cross section (45–100 GM at 860 nm),
high biocompatibility, and good photostability. They could specifically
stain lipid droplets at ultralow concentration (50 nM) in a short
time of 15 min. Such ultralow concentration is the lowest value for
lipid droplets staining in live cells reported so far. In
vitro and ex vivo two-photon imaging of
lipid droplets in live cells and live mice liver tissues were successfully
demonstrated. In addition, selective visualization of lipid droplets
in live mice liver tissues could be achieved at a depth of about 70
μm. These excellent properties render them as promising candidates
for investigating lipid droplet-associated physiological and pathological
processes in live biological samples.