pr500916m_si_002.zip (2.19 MB)
Shotgun Ecotoxicoproteomics of Daphnia pulex: Biochemical Effects of the Anticancer Drug Tamoxifen
dataset
posted on 2015-01-02, 00:00 authored by Myriam Borgatta, Céline Hernandez, Laurent Arthur Decosterd, Nathalie Chèvre, Patrice WaridelAmong
pollutants released into the environment by human activities,
residues of pharmaceuticals are an increasing matter of concern because
of their potential impact on ecosystems. The aim of this study was
to analyze differences of protein expression resulting from acute
(2 days) and middle-term (7 days) exposure of aquatic microcrustacean Daphnia pulex to the anticancer drug tamoxifen. Using a
liquid chromatography–mass spectrometry shotgun approach, about
4000 proteins could be identified, providing the largest proteomics
data set of D. pulex published up to now. Considering
both time points and tested concentrations, 189 proteins showed a
significant fold change. The identity of regulated proteins suggested
a decrease in translation, an increase in protein degradation and
changes in carbohydrate and lipid metabolism as the major effects
of the drug. Besides these impacted processes, which reflect a general
stress response of the organism, some other regulated proteins play
a role in Daphnia reproduction. These latter results
are in accordance with our previous observations of the impact of
tamoxifen on D. pulex reproduction and illustrate
the potential of ecotoxicoproteomics to unravel links between xenobiotic
effects at the biochemical and organismal levels. Data are available
via ProteomeXchange with identifier PXD001257.
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189 proteinspulex reproductionorganismal levelslipid metabolismstress responselatter resultsDaphnia pulex4000 proteinstime pointsproteomics dataBiochemical EffectsDaphnia reproductionmicrocrustacean Daphnia pulexAnticancer Drug TamoxifenAmong pollutantsprotein degradationxenobiotic effectsPXDanticancer drug tamoxifenprotein expressionShotgun Ecotoxicoproteomics
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