Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function
datasetposted on 07.03.2022, 17:34 authored by Susmita Ghosh, Swapneil Parikh, Mehar Un Nissa, Arup Acharjee, Avinash Singh, Dhruv Patwa, Prashant Makwana, Arundhati Athalye, Abhilash Barpanda, Malini Laloraya, Sanjeeva Srivastava, Firuza Parikh
A considerable section of males suffered from COVID-19, with many experiencing long-term repercussions. Recovered males have been documented to have compromised fertility, albeit the mechanisms remain unclear. We investigated the impact of COVID-19 on semen proteome following complete clinical recovery using mass spectrometry. A label-free quantitative proteomics study involved 10 healthy fertile subjects and 17 COVID-19-recovered men. With 1% false discovery rate and >1 unique peptide stringency, MaxQuant analysis found 1099 proteins and 8503 peptides. Of the 48 differentially expressed proteins between the healthy and COVID-19-recovered groups, 21 proteins were downregulated and 27 were upregulated in COVID-19-recovered males. The major pathways involved in reproductive functions, such as sperm–oocyte recognition, testosterone response, cell motility regulation, adhesion regulation, extracellular matrix adhesion, and endopeptidase activity, were downregulated in COVID-19-recovered patients according to bioinformatics analysis. Furthermore, the targeted approach revealed significant downregulation of semenogelin 1 and prosaposin, two proteins related to male fertility. Therefore, we demonstrate the alteration of semen proteome in response to COVID-19, thus disrupting the male reproductive function despite the patient’s clinical remission. Hence, to understand fertility-related biological processes triggered by this infection, a protracted evaluation of the consequences of COVID-19 in recovered men is warranted.
Read the peer-reviewed publication
mechanisms remain unclearmany experiencing longmajor pathways involvedfalse discovery ratetwo proteins relatedextracellular matrix adhesioncell motility regulationrecovered patients accordingmale reproductive functionadhesion regulationreproductive functionsmale fertility21 proteinsrecovered menrecovered malesrecovered groupsunderstand fertilitythus disruptingterm repercussionssemen proteomicssemen proteomeprotracted evaluationpatient ’males sufferedendopeptidase activityconsiderable sectioncompromised fertilityclinical remissionbioinformatics analysis8503 peptides