posted on 2020-10-13, 03:03authored byXinrong Zhou, Lei Zhou, Xinna Ge, Xin Guo, Jun Han, Yongning Zhang, Hanchun Yang
Porcine
deltacoronavirus (PDCoV) is an emergent enteropathogenic
coronavirus associated with swine diarrhea. Porcine small intestinal
epithelial cells (IPEC) are the primary target cells of PDCoV infection
in vivo. Here, isobaric tags for relative and absolute quantification
(iTRAQ) labeling coupled to liquid chromatography–tandem mass
spectrometry (LC-MS/MS) was used to quantitatively identify differentially
expressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. A total
of 78 DEPs, including 23 upregulated and 55 downregulated proteins,
were identified at 24 h postinfection. The data are available via
ProteomeXchange with identifier PXD019975. To ensure reliability of
the proteomics data, two randomly selected DEPs, the downregulated
anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced
protein with tetratricopeptide repeats 1 (IFIT1), were verified by
real-time PCR and Western blot, and the results of which indicate
that the proteomics data were reliable and valid. Bioinformatics analyses,
including GO, COG, KEGG, and STRING, further demonstrated that a majority
of the DEPs are involved in numerous crucial biological processes
and signaling pathways, such as immune system, digestive system, signal
transduction, RIG-I-like receptor, mTOR, PI3K-AKT, autophagy, and
cell cycle signaling pathways. Altogether, this is the first study
on proteomes of PDCoV-infected host cells, which shall provide valuable
clues for further investigation of PDCoV pathogenesis.