posted on 2024-02-02, 20:43authored byNaveed Ziari, Marc K. Hellerstein
Mass isotopomer distribution analysis
(MIDA) is an analytical
technique
that measures the synthesis rate of biological polymers using combinatorial
probabilities and stable isotope labeling. Over the past few decades,
this method has been developed and applied to a wide range of uses
that have increased our understanding of metabolism and the etiology
and monitoring of disease. There is currently no publicly available
piece of software for performing MIDA calculations in a targeted manner
without its functionality being limited to a specific use case. We
present a cross-platform Python graphical user interface implementation
for research to obtain kinetic parameters easily from stable-isotope
labeling studies and provide the code and user manual on GitHub.