pr5b00465_si_002.xls (2.05 MB)
Proteomic Analysis on Cercariae and Schistosomula in Reference to Potential Proteases Involved in Host Invasion of Schistosoma japonicum Larvae
dataset
posted on 2015-11-06, 00:00 authored by Mu Liu, Chuan Ju, Xiao-Feng Du, Hai-Mo Shen, Ji-Peng Wang, Jian Li, Xu-Min Zhang, Zheng Feng, Wei HuSchistosomiasis
is a parasitic zoonosis posing great threat to
human health. The infection is acquired by larval cercariae penetrating
host skin and transforming into juveniles, schistosomula. Proteolytic
enzymes secreted from the cercarial acetabular glands are known to
aid to the skin penetration, but molecular mechanisms remain largely
unclear. To profile the protein composition and identify potential
invasive proteases, we developed a new method for simulating cercarial
transformation and collecting schistosomula, and for the first time,
we compared the proteomes of Schistosoma japonicum cercariae and schistosomula by using in-gel shotgun proteomic analysis.
Totally, 1972 proteins were identified in association with ten main
biological processes based on Gene Ontology analysis; 46 proteases
were detected in cercariae, and among them, 25 proteases disappeared
after penetrated. Notably, leishmanolysins and serine and cysteine
proteases were found abundant but differentially expressed. Recombinant
serine protease SjCE2b and cysteine protease SjCB2 were produced and
used for validation of native proteins. Immunofluorescence and Western
blotting assays detected SjCE2b and SjCB2 in cercariae but not in
schistosomula, suggesting the two enzymes might be consumed upon skin
migration. Our data comprehensively chart the proteomic changes during
cercarial invasion, revealing the potential proteases involved, providing
a platform for the development of molecular anti-infection strategy.
History
Usage metrics
Categories
Keywords
Host Invasionsimulating cercarial transformation46 proteases25 proteasesSchistosoma japonicum LarvaeSchistosomiasisSjCE 2b1972 proteinsskin migrationProteolytic enzymesRecombinant serine protease SjCE 2bProteomic AnalysisPotential Proteasescercarial invasionSjCB 2cysteine proteasesskin penetrationSchistosoma japonicum cercariaeGene Ontology analysiscercarial acetabular glandsprotein compositioncysteine protease SjCB 2schistosomulahost skinproteomic changes
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC