jf7b05001_si_001.xlsx (48.49 kB)
Proteome-Level Analysis of Metabolism- and Stress-Related Proteins during Seed Dormancy and Germination in Gnetum parvifolium
dataset
posted on 2018-02-28, 00:00 authored by Ermei Chang, Nan Deng, Jin Zhang, Jianfeng Liu, Lanzhen Chen, Xiulian Zhao, M. Abbas, Zeping Jiang, Shengqing ShiGnetum parvifolium is a rich source of materials
for traditional medicines, food, and oil, but little is known about
the mechanism underlying its seed dormancy and germination. In this
study, we analyzed the proteome-level changes in its seeds during
germination using isobaric tags for relative and absolute quantitation.
In total, 1,040 differentially expressed proteins were identified,
and cluster analysis revealed the distinct time points during which
signal transduction and oxidation–reduction activity changed.
Gene Ontology analysis showed that “carbohydrate metabolic
process” and “response to oxidative stress” were
the main enriched terms. Proteins associated with starch degradation
and antioxidant enzymes were important for dormancy-release, while
proteins associated with energy metabolism and protein synthesis were
up-regulated during germination. Moreover, protein-interaction networks
were mainly associated with heat-shock proteins. Furthermore, in accord
with changes in the energy metabolism- and antioxidant-related proteins,
indole-3-acetic acid, Peroxidase, and soluble sugar content increased,
and the starch content decreased in almost all six stages of dormancy
and germination analyzed (S1–S6). The activity of superoxide
dismutase, abscisic acid, and malondialdehyde content increased in
the dormancy stages (S1–S3) and then decreased in the germination
stages (S4–S6). Our results provide new insights into G. parvifolium seed dormancy and germination at the proteome
and physiological levels, with implications for improving seed propagation.