Multinodal Acoustic Trapping Enables High Capacity
and High Throughput Enrichment of Extracellular Vesicles and Microparticles
in miRNA and MS Proteomics Studies
posted on 2021-02-16, 23:13authored byAxel Broman, Andreas Lenshof, Mikael Evander, Lotta Happonen, Anson Ku, Johan Malmström, Thomas Laurell
We report a new design of an acoustophoretic trapping device with
significantly increased capacity and throughput, compared to current
commercial acoustic trapping systems. Acoustic trapping enables nanoparticle
and extracellular vesicle (EV) enrichment without ultracentrifugation.
Current commercial acoustic trapping technology uses an acoustic single-node
resonance and typically operates at flow rates <50 μL/min,
which limits the processing of the larger samples. Here, we use a
larger capillary that supports an acoustic multinode resonance, which
increased the seed particle capacity 40 times and throughput 25–40
times compared to single-node systems. The resulting increase in capacity
and throughput was demonstrated by isolation of nanogram amounts of
microRNA from acoustically trapped urinary EVs within 10 min. Additionally,
the improved trapping performance enabled isolation of extracellular
vesicles for downstream mass spectrometry analysis. This was demonstrated
by the differential protein abundance profiling of urine samples (1–3
mL), derived from the non-trapped versus trapped urine samples.