ic500910z_si_004.cif (2.4 MB)

Modeling the Active Site of [NiFe] Hydrogenases and the [NiFeu] Subsite of the C-Cluster of Carbon Monoxide Dehydrogenases: Low-Spin Iron(II) Versus High-Spin Iron(II)

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posted on 16.06.2014, 00:00 by Katharina Weber, Özlen F. Erdem, Eckhard Bill, Thomas Weyhermüller, Wolfgang Lubitz
A series of four [S2Ni­(μ-S)2FeCp*Cl] compounds with different tetradentate thiolate/thioether ligands bound to the Ni­(II) ion is reported (Cp* = C5Me5). The {S2Ni­(μ-S)2Fe} core of these compounds resembles structural features of the active site of [NiFe] hydrogenases. Detailed analyses of the electronic structures of these compounds by Mössbauer and electron paramagnetic resonance spectroscopy, magnetic measurements, and density functional theory calculations reveal the oxidation states Ni­(II) low spin and Fe­(II) high spin for the metal ions. The same electronic configurations have been suggested for the Cred1 state of the C-cluster [NiFeu] subsite in carbon monoxide dehydrogenases (CODH). The Ni–Fe distance of ∼3 Å excludes a metal–metal bond between nickel and iron, which is in agreement with the computational results. Electrochemical experiments show that iron is the redox active site in these complexes, performing a reversible one-electron oxidation. The four complexes are discussed with regard to their similarities and differences both to the [NiFe] hydrogenases and the C-cluster of Ni-containing CODH.