pr070278r_si_004.xls (45 kB)
Microscale Solution Isoelectric Focusing as an Effective Strategy Enabling Containment of Hemeoglobin-Derived Products for High-Resolution Gel-Based Analysis of the Plasmodium falciparum Proteome
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posted on 2020-04-02, 17:32 authored by Niroshini Nirmalan, Fiona Flett, Tom Skinner, John E. Hyde, Paul F. G. SimsThe high hemeozoin (beta-hemeatin) content of Plasmodium falciparum lysates imposes severe
limitations on the analysis of the malarial proteome, in particular compromising the loading capacities
of two-dimensional gels. Here we report on the adaptation of a recently developed solution-phase
isoelectric focusing-based fractionation technique as a prefractionation strategy for efficient containment
of hemeoglobin-derived products and complexity reduction, to facilitate the high-resolution gel-based
quantitative analysis of plasmodial lysates.
Keywords: malaria • proteomics • serine hydroxymethyltransferase • two-dimensional gel electrophoresis •
quantitation
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gel-basedmalarial proteomeadaptationhemeoglobin-derived productsquantitationPlasmodium falciparum lysatesHigh-Resolution Gel-Based AnalysiselectrophoresiscapacitycontentHemeoglobin-Derived ProductsPlasmodium falciparum Proteomelimitationbeta-hemeatinserinecomplexity reductionhydroxymethyltransferaseplasmodial lysatesproteomicloading capacitiesgelMicroscale Solution IsoelectricEffective Strategy Enabling Containmentprefractionation strategyanalysissolution-phase isoelectric focusing-based fractionation techniquecontainmenthemeozoinKeyword
