posted on 2015-07-07, 00:00authored byShisheng Sun, Hui Zhang
Protein glycosylation is one of the
most important protein modifications.
Glycosylation site occupancy alteration has been implicated in human
diseases and cancers. However, current glycoproteomic methods focus
on the identification and quantification of glycosylated peptides
and glycosylation sites but not glycosylation occupancy or glycoform
stoichiometry. Here we describe a method for large-scale determination
of the absolute glycosylation stoichiometry using three independent
relative ratios. Using this method, we determined 117 absolute N-glycosylation
occupancies in OVCAR-3 cells. Finally, we investigated the possible
functions and the determinants for partial glycosylation.