Version 2 2022-03-17, 16:05Version 2 2022-03-17, 16:05
Version 1 2022-03-16, 12:37Version 1 2022-03-16, 12:37
dataset
posted on 2022-03-17, 16:05authored bySaifei Liu, Liyang Xie, Jiaxuan Su, Binnian Tian, Anfei Fang, Yang Yu, Chaowei Bi, Yuheng Yang
Stripe
rust is a widespread and harmful wheat disease caused by Puccinia striiformis f. sp. tritici (Pst) worldwide. Targeted metabolome and transcriptomics
analyses of CYR23 infected leaves were performed to identify the differential
metabolites and differentially expressed genes related to wheat disease
resistance. We observed upregulation of 33 metabolites involved in
the primary and secondary metabolism, especially for homogentisic
acid (HGA), p-coumaroylagmatine, and saccharopine.
These three metabolites were mainly involved in the phenylpropanoid
metabolic pathway, hydroxycinnamic acid amides pathway, and saccharopine
pathway. Combined with transcriptome data on non-compatible interaction,
the synthesis-related genes of these three differential metabolites
were all upregulated significantly. The gene regulatory network involved
in response to Pst infection was constructed, which
revealed that several transcription factor families including WRKYs,
MYBs, and bZIPs were identified as potentially hubs in wheat resistance
response against Pst. An in vitro test showed that HGA effectively inhibited the germination of stripe
rust fungus urediniospores and reduced the occurrence of wheat stripe
rust. The results of gene silencing and overexpression of HGA synthesis-related
gene 4-hydroxyphenylpyruvate dioxygenase proved that HGA was involved
in wheat disease resistance. These results provided a further understanding
of the disease resistance of wheat and indicated that HGA can be developed
as a potential agent against Pst.