posted on 2020-04-27, 08:29authored byCheng Wang, Yanlei Zhang, Jinjuan Tan, Bicheng Chen, Linxiao Sun
Severe acute pancreatitis (SAP) is
caused by complicated biological
factors, and revealing its complex pathogenesis by single-target analysis
is difficult. Systematic studies have developed slowly because extraction
of degradable pancreatic proteins exposed to multiple proteases is
challenging. We present integrated whole proteomic and phosphoproteomic
profiles of SAP rats based on a modified protein extraction strategy
with less protein degradation. Data-dependent acquisition (DDA) and
data-independent acquisition (DIA) strategies were applied to select
an appropriate method. Total 275 differentially expressed proteins
and 757 differentially expressed phosphorylated proteins were identified
by DIA-based quantitative proteomics. Several signal transduction
pathways, including the AMPK, MAPK, and PI3K-Akt pathways, were enriched
in SAP. Up-regulation of phosphorylated proteins involved in the process
of TNFA signaling and inflammatory response was also detected in SAP.
Our results improve the understanding of SAP development and progression.