pr3c00027_si_004.xlsx (26.83 kB)
High-Field Asymmetric Waveform Ion Mobility Spectrometry: Practical Alternative for Cardiac Proteome Sample Processing
dataset
posted on 2023-04-11, 23:33 authored by Lizhuo Ai, Aleksandra Binek, Simion Kreimer, Matthew Ayres, Aleksandr Stotland, Jennifer E. Van EykHeart tissue sample preparation for mass spectrometry
(MS) analysis
that includes prefractionation reduces the cellular protein dynamic
range and increases the relative abundance of nonsarcomeric proteins.
We previously described “IN-Sequence” (IN-Seq) where
heart tissue lysate is sequentially partitioned into three subcellular
fractions to increase the proteome coverage more than a single direct
tissue analysis by mass spectrometry. Here, we report an adaptation
of the high-field asymmetric ion mobility spectrometry (FAIMS) coupled
to mass spectrometry, and the establishment of a simple one step sample
preparation coupled with gas-phase fractionation. The FAIMS approach
substantially reduces manual sample handling, significantly shortens
the MS instrument processing time, and produces unique protein identification
and quantification approximating the commonly used IN-Seq method in
less time.
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three subcellular fractionspreviously described “includes prefractionation reducesheart tissue lysatesignificantly shortenssequentially partitionedsequence ”relative abundancequantification approximatingproteome coveragepractical alternativephase fractionationnonsarcomeric proteinsmass spectrometryless timecommonly used