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HILIC- and SCX-Based Quantitative Proteomics of Chlamydomonas reinhardtii during Nitrogen Starvation Induced Lipid and Carbohydrate Accumulation
dataset
posted on 2012-12-07, 00:00 authored by Joseph Longworth, Josselin Noirel, Jagroop Pandhal, Phillip C. Wright, Seetharaman VaidyanathanNitrogen starvation induced changes in carbohydrate and
lipid content is described in several algal species. Although these
phenotypic changes are desirable, such manipulations also significantly
deteriorate culture health, ultimately halting growth. To optimize
biofuel production from algae, it is desirable to induce lipid accumulation
without compromising cell growth and survival. In this study, we utilized
an 8-plex iTRAQ-based proteomic approach to assess the model alga Chlamydomonas reinhardtii CCAP 11/32CW15+ under nitrogen
starvation. First-dimension fractionation was conducted using HILIC
and SCX. A total of 587 proteins were identified (≥3 peptides)
of which 71 and 311 were differentially expressed at significant levels
(p < 0.05), during nitrogen stress induced carbohydrate
and lipid production, respectively. Forty-seven percent more changes
with significance were observed with HILIC compared to SCX. Several
trends were observed including increase in energy metabolism, decrease
in translation machinery, increase in cell wall production and a change
of balance between photosystems I and II. These findings point to
a severely compromised system where lipid is accumulated at the expense
of normal functioning of the organism, suggesting that a more informed
and controlled method of lipid induction than gross nutrient manipulation
would be needed for development of sustainable processes.
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lipid contentCCAPfindings pointculture healthChlamydomonas reinhardtiilipid productionSCXCarbohydrate AccumulationNitrogen starvationSeveral trendslipid inductioncell wall productionnitrogen stresslipid accumulationmanipulationHILICCWNitrogen Starvation Induced Lipidcell growthcarbohydratetranslation machinerynitrogen starvationenergy metabolismbiofuel productionIIalgal speciesphenotypic changes587 proteins
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