pr6b00567_si_004.xlsx (89.87 kB)
Download fileEndoplasmic Reticulum Chaperones Are Potential Active Factors in Thyroid Tumorigenesis
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posted on 2016-08-01, 00:00 authored by Elena Uyy, Viorel
I. Suica, Raluca M. Boteanu, Dana Manda, Ancuta E. Baciu, Corin Badiu, Felicia AntoheThe study aimed to
evaluate the proteomic changes in benign follicular
adenoma versus malignant follicular variant of papillary thyroid carcinoma.
Tumor and nontumor adjacent samples were analyzed by liquid nanochromatography
mass spectrometry, and protein abundance was evaluated by label-free
quantification. Western blotting and quantitative real-time polymerase
chain reaction were used to validate and complement the mass spectrometry
data. The results demonstrated deregulated expression of four endoplasmic
reticulum chaperones (78 kDa glucose-regulated protein, endoplasmin,
calnexin, protein disulfide-isomerase A4), glutathione peroxidase
3 and thyroglobulin, all of them involved in thyroid hormone synthesis
pathway. The altered tissue abundance of endoplasmic reticulum chaperones
in thyroid cancer was correlated with serum expression levels. The
identified proteins significantly discriminate between adenoma and
carcinoma in both thyroid tissue and corresponding sera. Data are
available via ProteomeXchange with identifier PXD004322.
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nanochromatography mass spectrometrytissue abundanceThyroid Tumorigenesisthyroid carcinomaPXDEndoplasmic Reticulum Chaperonesthyroid cancerthyroid tissueprotein abundanceadenomaendoplasmic reticulum chaperonespolymerase chain reactionproteomic changesmass spectrometry dataserum expression levelsthyroid hormone synthesis pathway