am0c08722_si_002.xyz (88.47 kB)
Dually Crosslinked Supramolecular Hydrogel for Cancer Biomarker Sensing
dataset
posted on 2020-08-06, 22:20 authored by Jie Li, Chendong Ji, Baozhong Lü, Maksim Rodin, Jan Paradies, Meizhen Yin, Dirk KucklingLysophosphatidic
acid (LPA) as the biomarker of early stage ovarian cancer is essentially
difficult to detect due to lack of target spots. A dually crosslinked
supramolecular hydrogel (DCSH) was developed to achieve sensing of
LPA, which acts as a competitive guest molecule triggering the responsive
crosslinking of the DCSH. Through this strategy, the surface plasmon
resonance combined with optical waveguide spectroscopy could be used
to quantitatively detect LPA with a responsive range covering physiological
conditions (in pure form as well as mimicking LPA plasma solution)
with high selectivity and sensitivity. LPA efficiently immerses into
the host molecule β-cyclodextrin (β-CD) up to a 1:2 ratio
by the competitive interaction mechanism, confirmed by one-dimensional
nuclear overhauser effect spectroscopy (1D NOESY), high-resolution
mass spectrometry (HRMS), isothermal titration calorimetry (ITC),
and computational simulation. Our method opens a new strategy to detect
biomarkers without target spots and provides a platform for surface
plasmon resonance (SPR)-based sensors measuring small molecules.