ac203404n_si_002.xls (38.5 kB)
Construction of a Natural Mucin Microarray and Interrogation for Biologically Relevant Glyco-Epitopes
dataset
posted on 2012-04-03, 00:00 authored by Michelle Kilcoyne, Jared
Q. Gerlach, Ronan Gough, Mary E. Gallagher, Marian Kane, Stephen D. Carrington, Lokesh JoshiMucins are the principal components of mucus, and mucin
glycosylation
has important roles in defense, microbial adhesion, immunomodulation,
inflammation, and cancer. Mucin expression and glycosylation are dynamic,
responding to changes in local environment and disease. Potentially
hundreds of heterogeneous glycans can substitute one mucin molecule,
and it is difficult to identify biologically accessible glyco-epitopes.
Thirty-seven mucins, from the reproductive and gastrointestinal (GI)
tracts of six species (bovine, ovine, equine, porcine, chicken, and
deer) and from two human-derived cell lines, were purified. Following
optimization of mucin printing and construction of a novel mucin microarray,
the glycoprofiles of the whole mucins on the microarray were compared
using a panel of lectins and one antibody. Accessible glyco-motifs
of GI mucins varied according to species and localization of mucin
origin, with terminal fucose, the sialyl T-antigen, and N-linked oligosaccharides identified as potentially important. The
occurrence of T- and sialyl T-antigen varied in bovine and ovine reproductive
tract mucins, and terminal N-acetylgalactosamine
(GalNAc) and sulfated carbohydrates were detected. This study introduces
natural mucin microarrays as an effective tool for profiling mucin
glyco-epitopes and highlights their potential for discovery of biologically
important motifs in bacterial–host interactions and fertility.