pr7b00701_si_011.xlsx (116.31 kB)
Comprehensive Mass Spectrometric Survey of Streptococcus pyogenes Subcellular Proteomes
dataset
posted on 2017-11-21, 00:00 authored by Laura Wilk, Lotta Happonen, Johan Malmström, Heiko HerwaldStreptococcus pyogenes is a major global health
burden causing a wide variety of diseases. Because a vaccine against
this bacterium is still lacking, vaccine candidates or antimicrobial
therapies are urgently needed. Here we use an invasive and clinically
relevant streptococcal M1 serotype to characterize the bacterial proteome
in-depth. An elaborate fractionation technique is employed to separate
the different cell fractions, followed by shotgun mass-spectrometry
analysis, allowing us to confirm the expression of nearly two-thirds
(1022) of the 1690 open reading frames predicted for the streptococcal
M1 reference proteome. In contrast with other studies, we present
the entire isolated membrane proteome, which opens up a whole new
source for drug targets. We show both the unique and most prevalent
proteins for each cellular fraction and analyze the presence of predicted
cell-wall-anchored proteins and lipoproteins. With our approach, we
also identify a variety of novel proteins whose presence has not been
reported in previous proteome studies. Proteins of interest, potential
virulence factors, and drug or vaccine targets are discussed for each
cellular fraction. Overall, the results of this work represent the
so-far widest proteomic approach to characterize the protein composition
and localization in S. pyogenes.
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membrane proteomeproteomic approachdrug targetsvaccine targetscell-wall-anchored proteinspresencevarietyshotgun mass-spectrometry analysisfractionation techniquecell fractionshealth burdenComprehensive Mass Spectrometric Surveystreptococcal M 1 reference proteomereading framesstreptococcal M 1 serotypeproteome studiesantimicrobial therapiesStreptococcus pyogenes Subcellular Proteomes Streptococcus pyogenesprotein compositionvaccine candidatesvirulence factorsnovel proteins
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