pr8b00355_si_002.xlsx (20.88 kB)
Characterization of the Blister Fluid Proteome for Pediatric Burn Classification
dataset
posted on 2018-12-06, 00:00 authored by Tuo Zang, Leila Cuttle, Daniel A. Broszczak, James A. Broadbent, Catherine Tanzer, Tony J. ParkerBlister
fluid (BF) is a novel and viable research matrix for burn
injury study, which can reflect both systemic and local microenvironmental
responses. The protein abundance in BF from different burn severities
were initially observed using a 2D SDS-PAGE approach. Subsequently,
a quantitative data independent acquisition (DIA) method, SWATH, was
employed to characterize the proteome of pediatric burn blister fluid.
More than 600 proteins were quantitatively profiled in 87 BF samples
from different pediatric burn patients. These data were correlated
with clinically assessed burn depth and time until complete wound
re-epithelialization through several different statistical analyses.
Several proteins from these analyses exhibited significant abundance
change between different burn depth or re-epithelialization groups,
and can be considered as potential biomarker candidates. Further gene
ontology (GO) enrichment analysis of the significant proteins revealed
the most significant burn related biological processes (BP) that are
altered with burn depth, including homeostasis and oxygen transport.
However, for wounds with re-epithelialization times more or less than
21 days, the significant GO annotations were related to enzyme activity.
This quantitative proteomics investigation of burn BF may enable objective
classification of burn wound severity and assist with clinical decision-making.
Data are available via ProteomeXchange with identifier PXD011102.
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gene ontologyBPwound re-epithelializationre-epithelialization timesenzyme activityenrichment analysisSWATHbiomarker candidatesPediatric Burn Classification Blister fluidwound severityanalysesprotein abundanceinjury studyBlister Fluid Proteome600 proteins2 D SDS-PAGE approachre-epithelialization groupsblister fluidobjective classificationSeveral proteinsoxygen transportDIAmicroenvironmental responsesproteomics investigationresearch matrix87 BF samplesPXD21 daysdataabundance changedepth
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