pr9b00351_si_002.xlsx (248.76 kB)
Cell Type-Specific Expression of Testis Elevated Genes Based on Transcriptomics and Antibody-Based Proteomics
Version 2 2019-09-30, 21:43
Version 1 2019-08-29, 18:03
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posted on 2019-08-29, 18:03 authored by Charles Pineau, Feria Hikmet, Cheng Zhang, Per Oksvold, Shuqi Chen, Linn Fagerberg, Mathias Uhlén, Cecilia LindskogOne of the most complex organs in
the human body is the testis,
where spermatogenesis takes place. This physiological process involves
thousands of genes and proteins that are activated and repressed,
making testis the organ with the highest number of tissue-specific
genes. However, the function of a large proportion of the corresponding
proteins remains unknown and testis harbors many missing proteins
(MPs), defined as products of protein-coding genes that lack experimental
mass spectrometry evidence. Here, an integrated omics approach was
used for exploring the cell type-specific protein expression of genes
with an elevated expression in testis. By combining genome-wide transcriptomics
analysis with immunohistochemistry, more than 500 proteins with distinct
testicular protein expression patterns were identified, and these
were selected for in-depth characterization of their in situ expression in eight different testicular cell types. The cell type-specific
protein expression patterns allowed us to identify six distinct clusters
of expression at different stages of spermatogenesis. The analysis
highlighted numerous poorly characterized proteins in each of these
clusters whose expression overlapped with that of known proteins involved
in spermatogenesis, including 88 proteins with an unknown function
and 60 proteins that previously have been classified as MPs. Furthermore,
we were able to characterize the in situ distribution
of several proteins that previously lacked spatial information and
cell type-specific expression within the testis. The testis elevated
expression levels both at the RNA and protein levels suggest that
these proteins are related to testis-specific functions. In summary,
the study demonstrates the power of combining genome-wide transcriptomics
analysis with antibody-based protein profiling to explore the cell
type-specific expression of both well-known proteins and MPs. The
analyzed proteins constitute important targets for further testis-specific
research in male reproductive disorders.
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Testis Elevated Genestestis-specific researchgenome-wide transcriptomics analysisprotein levelstesticular protein expression patternstesticular cell typesomics approachcell Type-Specific Expressionmass spectrometry evidenceAntibody-Based Proteomicsexpression levelsprotein-coding genescell type-specific protein expressioncell type-specific protein expression patterns60 proteinstestis harborsRNAtissue-specific genestestis-specific functionsMP500 proteinscell type-specific expression88 proteins
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