Building Up Key Segments of N-Glycans in the Gas Phase: Intrinsic Structural Preferences of the α(1,3) and α(1,6) Dimannosides
datasetposted on 2006-02-15, 00:00 authored by Pierre Çarçabal, Isabel Hünig, David P. Gamblin, Bo Liu, Rebecca A. Jockusch, Romano T. Kroemer, Lavina C. Snoek, Anthony J. Fairbanks, Benjamin G. Davis, John P. Simons
The intrinsic conformer specific vibrational spectra of two important subunits of the core pentasaccharide of N-linked glycans, the α(1,3) and α(1,6) dimannosides, have been recorded in the gas phase. Coupling these measurements with a computational exploration of their conformational landscapes has enabled their conformational assignment and has identified characteristic vibrational signatures associated with particular conformational familiesincluding those that do or do not display inter-ring hydrogen bonding across the glycosidic linkage. In addition, the IR spectra of the monosaccharide moieties provide benchmarks, through which the conformational assignments can be refined. This introduces a general concept of modularity and secondary structure in oligosaccharidesessential for the success of similar studies on larger oligosaccharides in the future.
Read the peer-reviewed publication
Preferencesmodularitydimannosidevibrational signaturesconceptmeasurementlandscapeglycosidic linkagemonosaccharide moietiesGlycanPhasesubunitIntrinsicStructuraloligosaccharidevibrational spectrabenchmarkassignmentglycanconformerIR spectraDimannosidegas phaseKey Segmentsfutureexplorationcore pentasaccharidedisplay