An [Fe₄S₄]³⁺–Alkyl Cluster Stabilized by an Expanded Scorpionate Ligand

Alkyl-ligated iron–sulfur clusters in the [Fe₄S₄]³⁺ charge state have been proposed as short-lived intermediates in a number of enzymatic reactions. To better understand the properties of these intermediates, we have prepared and characterized the first synthetic [Fe₄S₄]³⁺–alkyl cluster. Isolation of this highly reactive species was made possible by the development of an expanded scorpionate ligand suited to the encapsulation of cuboidal clusters. Like the proposed enzymatic intermediates, this synthetic [Fe₄S₄]³⁺–alkyl cluster adopts an S = ¹/₂ ground state with g_iso > 2. Mössbauer spectroscopic studies reveal that the alkylated Fe has an unusually low isomer shift, which reflects the highly covalent Fe–C bond and the localization of Fe³⁺ at the alkylated site in the solid state. Paramagnetic ¹H NMR studies establish that this valence localization persists in solution at physiologically relevant temperatures, an effect that has not been observed for [Fe₄S₄]³⁺ clusters outside of a protein. These findings establish the unusual electronic-structure effects imparted by the strong-field alkyl ligand and lay the foundation for understanding the electronic structures of [Fe₄S₄]³⁺–alkyl intermediates in biology.