American Chemical Society
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A New Lysosome-Targetable Turn-On Fluorogenic Probe for Carbon Monoxide Imaging in Living Cells

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posted on 2018-01-22, 00:00 authored by Koushik Dhara, Somenath Lohar, Ayan Patra, Priya Roy, Swadhin Kumar Saha, Gobinda Chandra Sadhukhan, Pabitra Chattopadhyay
A lysosome-targetable fluorogenic probe, LysoFP-NO2, was designed and synthesized based on a naphthalimide fluorophore that can detect selectively carbon monoxide (CO) in HEPES buffer (pH 7.4, 37 °C) through the transformation of the nitro group into an amino-functionalized system in the presence of CO. LysoFP-NO2 triggered a “turn-on” fluorescence response to CO with a simultaneous increase of fluorescence intensity by more than 75 times. The response is selective over a variety of relevant reactive nitrogen, oxygen, and sulfur species. Also, the probe is an efficient candidate for monitoring changes in intracellular CO in living cells (MCF7), and the fluorescence signals specifically localize in the lysosome compartment.