American Chemical Society
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A Highly Selective Low-Background Fluorescent Imaging Agent for Nitric Oxide

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posted on 2010-09-29, 00:00 authored by Youjun Yang, Stephanie K. Seidlits, Michelle M. Adams, Vincent M. Lynch, Christine E. Schmidt, Eric V. Anslyn, Jason B. Shear
We introduce a novel sensing mechanism for nitric oxide (NO) detection with a particular easily synthesized embodiment (NO550), which displays a rapid and linear response to NO with a red-shifted 1500-fold turn-on signal from a dark background. Excellent selectivity was observed against other reactive oxygen/nitrogen species, pH, and various substances that interfere with existing probes. NO550 crosses cell membranes but not nuclear membranes and is suitable for both intra- and extracellular NO quantifications. Good cytocompatibility was found during in vitro studies with two different cell lines. The high specificity, dark background, facile synthesis, and low pH dependence make NO550 a superior probe for NO detection when used as an imaging agent.