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Download fileA Genome-Wide Regulator–DNA Interaction Network in the Human Pathogen Mycobacterium tuberculosis H37Rv
dataset
posted on 07.09.2012, 00:00 by Jumei Zeng, Tao Cui, Zheng-Guo HeTranscription regulation translates static genome information
to dynamic cell behaviors, making it central to understand how cells
interact with and adapt to their environment. However, only a limited
number of transcription regulators and their target genes have been
identified in the pathogen Mycobacterium tuberculosis, which has greatly impeded our understanding of its pathogenesis
and virulence. In this study, we constructed a genome-wide transcription
regulatory network of M. tuberculosis H37Rv using
a high-throughput bacterial one-hybrid technique. A transcription
factor skeleton network was derived on the basis of the identification
of more than 5400 protein–DNA interactions. Our findings further
highlight the regulatory mechanism of the mammalian cell entry 1 (mce1) module, which includes mce1R and
the mce1 operon. Mce1R was linked to global negative
regulation of cell growth, but was found to be positively regulated
by the dormancy response regulator DevR. Expression of the mce1 operon was shown to be negatively regulated by the
virulence regulator PhoP. These findings provide important new insights
into the molecular mechanisms of several mce1 module-related
hypervirulence phenotypes of the pathogen. Furthermore, a model of mce1 module-centered signal circuit for dormancy regulation
in M. tuberculosis is proposed and discussed.
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Human Pathogen Mycobacterium tuberculosis H 37RvTranscription regulationMce 1Rtranscription factor skeleton networktarget genesdormancy regulationpathogen Mycobacterium tuberculosisvirulence regulator PhoPtranscription regulatorscell entry 1cell growthmce 1Rcell behaviorsdormancy response regulator DevRgenome informationtuberculosis H 37Rvmce 1 operon