am5b06874_si_002.avi (2.9 MB)
In Vivo Capture of Circulating Tumor Cells Based on Transfusion with a Vein Indwelling Needle
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posted on 2015-09-16, 00:00 authored by Hongyan Zhang, Zhenzhen Jia, Chuanchen Wu, Liguo Zang, Guiwen Yang, Zhenzhen Chen, Bo TangDetection
of circulating tumor cells (CTCs) could be used as a
“liquid biopsy” for tracking the spread of cancer. In vitro detection methods based on blood sampling and in vitro CTC capture often suffer from the small sampling
volume and sampling error. Here, the in vivo capture
of CTCs based on transfusion with a surface-modified vein indwelling
needle is proposed. When the needle was applied to transfusion in
the vein, the simultaneous capture of CTCs was performed. To investigate
the actual capture efficiency of the in vivo capture
method, labeled MCF-7 cells were directly injected into the veins
of rabbits, wild type mice, and nude mice and could be successfully
captured. Two of 5 MCF-7 cells injected into the veins of nude mice
were successfully captured. To investigate the CTC capture of mouse
tumor model and compare with the in vitro method,
mice were subcutaneous inoculated with metastatic 4T1 cells. Seven
and 21 days after inoculation, CTCs were captured for the first time
using in vivo and in vitro methods,
respectively. This predicted that the in vivo method
could be more suitable for use of early diagnosis of cancer than the in vitro method. As CTC capture can be performed at the
same time as transfusion and does not cause further bodily harm, it
would be easily accepted by patients. This efficient, simple, and
less damaging method involving the use of a vein indwelling needle
could be popularized easily in the clinic.