es7b04152_si_001.pdf (1.52 MB)
In Situ Stimulation of Thiocyanate Biodegradation through Phosphate Amendment in Gold Mine Tailings Water
journal contribution
posted on 2017-10-24, 00:00 authored by Mathew
P. Watts, Han M. Gan, Lee Y. Peng, Kim-Anh Lê Cao, John W. MoreauThiocyanate (SCN–) is a contaminant requiring
remediation in gold mine tailings and wastewaters globally. Seepage
of SCN–-contaminated waters into aquifers can occur
from unlined or structurally compromised mine tailings storage facilities.
A wide variety of microorganisms are known to be capable of biodegrading
SCN–; however, little is known regarding the potential
of native microbes for in situ SCN– biodegradation, a remediation option that is less costly than engineered
approaches. Here we experimentally characterize the principal biogeochemical
barrier to SCN– biodegradation for an autotrophic
microbial consortium enriched from mine tailings, to arrive at an
environmentally realistic assessment of in situ SCN– biodegradation potential. Upon amendment with phosphate,
the consortium completely degraded up to ∼10 mM SCN– to ammonium and sulfate, with some evidence of nitrification of
the ammonium to nitrate. Although similarly enriched in known SCN–-degrading strains of thiobacilli, this consortium
differed in its source (mine tailings) and metabolism (autotrophy)
from those of previous studies. Our results provide a proof of concept
that phosphate limitation may be the principal barrier to in situ SCN– biodegradation in mine tailing
waters and also yield new insights into the microbial ecology of in situ SCN– bioremediation involving
autotrophic sulfur-oxidizing bacteria.