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Xylanase Immobilized on Novel Multifunctional Hyperbranched Polyglycerol-Grafted Magnetic Nanoparticles: An Efficient and Robust Biocatalyst
journal contribution
posted on 2015-08-25, 00:00 authored by Amir Landarani-Isfahani, Asghar Taheri-Kafrani, Mina Amini, Valiollah Mirkhani, Majid Moghadam, Asieh Soozanipour, Amir RazmjouAlthough several strategies are now
available for immobilization
of enzymes to magnetic nanoparticles for bioapplications, little progresses
have been reported on the use of dendritic or hyperbranched polymers
for the same purpose. Herein, we demonstrated synthesis of magnetic
nanoparticles supported hyperbranched polyglycerol (MNP/HPG) and a
derivative conjugated with citric acid (MNP/HPG-CA) as unique and
convenient nanoplatforms for immobilization of enzymes. Then, an important
industrial enzyme, xylanase, was immobilized on the nanocarriers to
produce robust biocatalysts. A variety of analytical tools were used
to study the morphological, structural, and chemical properties of
the biocatalysts. Additionally, the results of biocatalyst systems
exhibited the substantial improvement of reactivity, reusability,
and stability of xylanase due to this strategy, which might confer
them a wider range of applications.