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Xenohybrid Bone Graft Containing Intrinsically Disordered Proteins Shows Enhanced In Vitro Bone Formation
journal contribution
posted on 2020-03-13, 14:03 authored by Hao Zhu, Manuel Gomez, Jun Xiao, Giuseppe Perale, Felice Betge, Ståle Petter Lyngstadaas, Håvard Jostein HaugenBone
defects are a significant health problem worldwide, as bone is the
second-most transplanted tissue after blood. Although a myriad of
bone grafts (BGs) have been used to treat bone repairs, none of them
possesses all the desirable characteristics. An approach to improve
BGs is to add bio-active components, however often difficult as BG
production may disrupt the biological activities of such molecules.
Here, we present a composite xenohybrid BG, SmartBonePep, with a type
of biomolecule inspired by intrinsically disordered proteins (IDPs).
These synthetic peptides (named P2 and P6) are physically entrapped
into the polymer matrix of the composite BG. The effects of SmartBonePep
on human osteoblasts were tested. Results showed that SmartBonePep
enhanced proliferation and osteogenic effects. In order to verify
the bioactivity of P2 and P6, these peptides were tested indirectly
by being added to cell culture media too. Here, P2 or P6 exhibited
promoting effects on osteogenic-related gene expressions. In this
study, we showed highly effective osteoinductive synthetic peptides
P2 or P6, which possess proline-rich and intrinsically disordered
structural characters. This use of IDPs may provide promising bone
enhancement biomolecules for clinical usage.