es0c00099_si_001.pdf (1.83 MB)
Visualized Metabolic Disorder and Its Chemical Inducer in Wild Crucian Carp from Taihu Lake, China
journal contribution
posted on 2020-03-05, 18:11 authored by Shixiong Gao, Hang Liu, Hong Chang, Zhaobin Zhang, Jianying Hu, Shu Tao, Yi WanA variety
of anthropogenic chemicals can disrupt the equilibrium
of intrinsic biological metabolites in organisms, leading to metabolic
disorders and an increased risk of metabolic syndromes. However, exposure
to pollutants that induce metabolic disorders in wildlife as a cause
of adverse effects is unknown. In this study, approximately 3108 compounds,
including 11 groups of metabolites and 388 pollutants, were simultaneously
identified in the blood of wild crucian carp (Carassius
auratus) captured in three bays of Taihu Lake, China.
A visualized network linking thousands of co-regulated metabolites
was automatically produced for the screened signals. This comprehensive
view of the differences in blood metabolite profiles in carp from
the north and south bays showed that triglycerides (TGs) were the
intrinsic molecules most affected by differing environmental pollution
in each bay. The regional differences in metabolite profiles were
linked to exposure to screened perfluorinated compounds that displayed
corresponding regional differences in concentrations and effects on
TGs in in vivo exposure tests. Perfluoroundecanoic
acid (PFUnDA) was the key pollutant responsible for the variation
in blood TGs in wild crucian carp, and exposure to PFUnDA resulted
in extremely high biological activity on lipid deposition in the liver
tissues of crucian carp at environmental levels.