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Verification of the Role of MlrC in Microcystin Biodegradation by Studies Using a Heterologously Expressed Enzyme
journal contribution
posted on 2012-06-18, 00:00 authored by Dariusz Dziga, Marcin Wasylewski, Adam Szetela, Oliwia Bocheńska, Benedykt WladykaThe MlrC protein from Sphingomonas ACM-3962
strain
was heterologously expressed in Escherichia coli strain
BL21(DE3) and purified to investigate participation of this enzyme
in the biodegradation of two microcystin variants. In contrast with
previous reports, our results indicated that MlrC cleaves linear microcystins,
thus shedding new light on the role of MlrC enzyme in microcystin
biodegradation.