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Vegetable Signatures Derived from Human Urinary Metabolomic Data in Controlled Feeding Studies
journal contribution
posted on 2018-12-05, 00:00 authored by Ke-Shiuan Lynn, Mei-Ling Cheng, Hsin-Chou Yang, Yu-Jen Liang, Mei-Jyh Kang, Fong-Ling Chen, Ming-Shi Shiao, Wen-Harn PanExamination
of changes in urinary metabolomic profiles after vegetable
ingestion may lead to new methods of assessing plant food intake.
To this regard, we developed a proof-of-principle methodology to identify
urinary metabolomic signatures for spinach, celery, and onion. Three
feeding studies were conducted. In the first study, healthy individuals
were fed with spinach, celery, onion, and no vegetables in four separate
experiments with pooled urinary samples for metabolite discovery.
The same protocol was used to validate the finding at the individual
level in the second study and when feeding all three vegetables simultaneously
in the third study. An LC–MS-based metabolomics approach was
adopted to search for indicative metabolites from urine samples collected
during multiple time periods before and after the meal. Consequently,
a total of 1, 9, and 3 nonoverlapping urinary metabolites were associated
with the intake of spinach, celery, and onion, respectively. The PCA
signature of these metabolites followed a similar “time cycle”
pattern, which maximized at approximately 2–4 h after intake.
In addition, the metabolite profiles for the same vegetable were consistent
across samples, regardless of whether it was consumed individually
or in combination. The developed methodology along with the identified
urinary metabolomic signatures were potential tools for assessing
plant food intake.